MOLigo Design

Ligation conditions
Oligo concentration
M
Monovalent salt concentration
M
Divalent salt concentration
M
Nucleotide triphosphates (dNTPs)
M
Specific region Tm(95%)
°C
MOLigo parameters
Max specific region length
bp
Min specific region length
bp
Secondary structure boundary Tm
°C
Range of Tm (+/-)
°C
Delta Tm of specific regions
°C
LNA base parameters
Maximum trim 5'
bp
Maximum trim 3'
bp
Minimal trim length
bp
Optimal trim length
bp
Maximum LNA bases
bp
Other options
MOL-PCR version: Classic Reverse Do not introduce LNA bases if Tm of region is out of range
Forward universal primer
5'- -3'
Reverse universal primer
5'- -3'